Metadata
| Sample description | Asparagus racemosus |
|---|---|
| Metabolite extraction protocol | The samples were pulverized with liquid nitrogen. Around 100 mg of powder was weighed and mixed with 1 ml of ice-cold extraction solution (75% aqueous MeOH+0.1% FA). Sonicated the samples in water bath at room temperature with maximum frequency of 40 kHz for 15 min. Centrifugation was performed at 12,000 rpm for 15 min. The supernatant was filtered using sterile 0.22 µm filter and required quanity of filtrate to be used for subsequent mass spectrometry analysis |
| Instrument used | Waters Xevo G2-XS QTOF-MS |
| Instrument type | UPLC-QTOF-MSE |
| Ionization | ESI |
| Ionization mode | Positive |
| Column used | Acquity UPLC BEH C18 1.7µm |
| Run time | 18 min |
| Mobile phase | (A) Deioinized water with 0.1% formic acid (B) Acetonitrile with 0.1% formic acid |
| Gradient program | 10% (B) at 0-1 min, 10-90%(B) at 1-13 min, 90-95%(B) at 13-14 min, 95-10%(B) at 14-18 min |
| Flow rate used | 0.4 ml/min |
| Collision energy | ramp 20-40 eV |